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KMID : 0613820080180040537
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Journal of Life Science
2008 Volume.18 No. 4 p.537 ~ p.541
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The Effect of Haptoglobin on Expression of Inflammatory Cytokines in 3T3-L1 Preadipocytes
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Cho Jin-Kyung
Kim Nam-Hoon
Oh Mi-Kyung
Park Seon-Joo
Kim In-Sook
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Abstract
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White adipose tissue is now recognized as an important endocrine organ which secretes various signal factors and proteins termed ’adipokine’. Haptoglobin (Hp), which has been known as an acute phase protein, belongs to the adipokine. However, the function of Hp in adipose tissue remains unclear. To verify the role of Hp in preadipocytes, in this study, 3T3-L1 preadipocyte cells were stably transfected with human Hp gene and Hp-overexpressing cells were made. The Hp had no effect on cell growth of preadipocytes. By RT-PCR and Western blot analysis, the Hp inhibited gene expression of IL-6 and COX-2 and enhanced HO-1 synthesis in preadipocytes. Moreover, invasion assay showed the Hp suppressed migration of monocytes to preadipocytes. These findings suggest that the Hp may inhibit an inflammatory reaction in adipose tissue by regulating the expressions of pro-inflammatory and anti-inflammatory mediators, and by repressing monocytes/macrophages infiltration.
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KEYWORD
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Haptoglobin, cyclooxygenase-2, interleukin-6, heme oxygenase-1, 3T3-L1 preadipocytes
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